Searching for a Bacteriophage Lysin to Treat Corynebacterium bovis in Immunocompromised Mice
نویسندگان
چکیده
منابع مشابه
PlyC: a multimeric bacteriophage lysin.
Lysins are murein hydrolases produced by bacteriophage that act on the bacterial host cell wall to release progeny phage. When added extrinsically in their purified form, these enzymes produce total lysis of susceptible Gram-positive bacteria within seconds, suggesting a unique antimicrobial strategy. All known Gram-positive lysins are produced as a single polypeptide containing a catalytic act...
متن کاملUse of a Bacteriophage Lysin to Identify a Novel Target for Antimicrobial Development
We identified an essential cell wall biosynthetic enzyme in Bacillus anthracis and an inhibitor thereof to which the organism did not spontaneously evolve measurable resistance. This work is based on the exquisite binding specificity of bacteriophage-encoded cell wall-hydrolytic lysins, which have evolved to recognize critical receptors within the bacterial cell wall. Focusing on the B. anthrac...
متن کاملPurification of Streptococcus group C bacteriophage lysin.
A simple procedure for the purification of Streptococcus group C phage lysin to apparent homogeneity is described. The electrophoretically pure, enzymatically stable polypeptide of 98,000 molecular weight converted Streptococcus (groups A, F, and H) cells into spheroplasts within 5 min at 0 degrees C or within less than a minute at 37 degrees C.
متن کاملEffect of bacteriophage lysin on lysogens.
OBJECTIVE To study the effect of phage lysin on the growth of lysogens. METHODS Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18-24 hou...
متن کاملDetection of Corynebacterium bovis infection in athymic nude mice from a research animal facility in Korea
Corynebacterium (C.) bovis infection in nude mice causes hyperkeratosis and weight loss and has been reported worldwide but not in Korea. In 2011, nude mice from an animal facility in Korea were found to have white flakes on their dorsal skin. Histopathological testing revealed that the mice had hyperkeratosis and Gram-positive bacteria were found in the skin. We identified isolated bacteria fr...
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ژورنال
عنوان ژورنال: Comparative Medicine
سال: 2020
ISSN: 1532-0820
DOI: 10.30802/aalas-cm-19-000096